The normal bacteria found on the skin of humans are capable of producing characteristic foul odors, especially on the foot and in the axillary regions.1,11 In order to obtain a samping of normal bacteria on the foot, untreated 75 percent ORLON/25 percent nylon socks were worn by laboratory peronnel during a routine workday, removed at home, sealed in plastic bags, and returned to the microbiology lab the following day. The bacteria were extracted from the socks in a liquid growth medium, isolated, and identified. The bacterial isolates obtained in the study are listed in table I (see above).
The bacteria represent a spectrum of Gram positive and Gram negative organisms capable of producing odors on textiles in contact with the skin. Figure I shows the correlation of increase in odor with increase in bacterial isolates.
Once the odor-causing bacteria were isolated, it as necessary to determine if treatment with AEM 5700 Antimicrobial would inhibit the growth of these microorganisms on the socks. ORLON/nylon socks treated with AEM 5700 Antimicrovial were supplied by Bulinton Socks/Adler. The socks were tested against each of the bacterial isolates frm the foot acccording to AATCC Test Method 100 - 197712 modified to include 0.1 percent triton X-100 saline inoculum. The results of this testing are in Table II (see above).
The socks treated with AEM 5700 Antimicrobial were effective in inhibiting the growth of the odor causing bacteria on the sock.
A biostatic finish on a textile such as a sock must be durable to repeated home laundering if the benefit of the treatment is to be realized for the life of the article. Table III (see above) shows the antimicrobial activity of the socks following repeated laundering.
The antimicrobial activity of the treated sockks was not reduced after 40 laundering cycles. Treated socks were machine washed through in various laundry detergents in the home. Residual antimicrobial activity was measured.
The antimicrobial activity of the treated socks was durable for the 10 laundering cycles in the detergents tested.
The in Vitro tests indicated that socks treated with AEM 5700 Antimicrobial do have a durable antimicrobial finish. To study the practicality of inhibiting the growth of odor-causing bacteria on socks under actual use conditions, an in vivo organoleptic test was completed.
Burlington Socks/Adler supplied untreated ORLON/nylon control socks and socks treated with AEM 5700 Antimicrobial to an independent test laboratory to compare the odors of socks following normal wear by male panelists. The socks included unwashed and washed (ten laundry cycles) control and treated socks.
The male panelists were each given a control and treated sock daily during the test period. Each sock was to be worn on a specific foot. At the end of the workday , panelists reported, panelists reported to the lab to remove the socks, seal them in plastic bags, and receive socks for the next day. Odor evaluations were made by four odor judges and new sheets were used every day of the evaluation. The odor grading scale was O to 10("no odor" to "very intense and disagreeable odor").
There was a significant reduction in odor of washed treated socks compared to washed control socks at the 99 percent significance level. The difference in results between washed and unwashed treated socks when compared to controls is explained by the presence of nonsubstantive process chemicals such as dye carriers, softeners and wetting agents which are removed upon washing. The presence of these chemicals may:
- Impart hydrophobic character to the socks which inhibits intimate contact of microorganisms with the testily surface, thus reducing effectiveness.
- Impart a pleasant, new-clothing odor which can mask unpleasant odors and is similar in action to a deodorant.
- Impart a temporary, leachable antimicrobial activity, usually due to the quaternary nature of most textile finishes such as softeners and antistats.
The results of this comparative evaluation show that the AEM 5700 antimicrobial agent is bonded to the textile (sock) and is not removed by repeated laundering. In contrast to untreated controls, repeated washing does not destroy the antimicrobial activity nor odor reduction of the treated socks.
III. Application of AEM 5700 Antimicrobial to Other Textiles
Microbiological evaluations have been completed on a variety of other textiles treated with AEM 5700 Antimicrobial.
The fabrics include a diverse spectrum of fiber types and blends. They were treated against a broad spectrum of microorganisms including odor-causing bacteria, and bacteria and fungi which cause rot and mildew. In all cases, the treated textiles exhibited inhibition of growth of the microorganisms on the fabric, thus preventing rot and mildew, and reducing the production of foul odors.
Conclusion
Chemical bonding of an organosilicon quaternary ammonium compound to textile substrates results in affective reduction of odor-causing microorganisms under actual in-use conditions of wear. Effectiveness is not reduced by laundering.
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